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Reference Number BB/W003449/1
Title Creating and comprehending the circuitry of life: precise biomolecular design of multi-centre redox enzymes for a synthetic metabolism
Status Started
Energy Categories OTHER CROSS-CUTTING TECHNOLOGIES or RESEARCH 5%;
NOT ENERGY RELATED 95%;
Research Types Basic and strategic applied research 100%
Science and Technology Fields BIOLOGICAL AND AGRICULTURAL SCIENCES (Biological Sciences) 50%;
PHYSICAL SCIENCES AND MATHEMATICS (Chemistry) 50%;
UKERC Cross Cutting Characterisation Not Cross-cutting 100%
Principal Investigator Dr R A (Ross ) Anderson
No email address given
Biochemistry
University of Bristol
Award Type Research Grant
Funding Source BBSRC
Start Date 01 May 2022
End Date 30 April 2027
Duration 60 months
Total Grant Value £3,919,670
Total Project Value £3,919,670
Industrial Sectors
Region South West
Programme
 
Investigators Principal Investigator Dr R A (Ross ) Anderson , Biochemistry, University of Bristol (100.000%)
Web Site
Objectives Objectives not supplied
Abstract A defining characteristic of life is the requirement of energy from an external source; we eat, plants absorb light. To maximize the energy gained from the food that we and all oxygen-breathing organisms consume, oxygen is converted to water as a final step and carbon dioxide is released. The oxygen in this equation arises from plants as they convert water, carbon dioxide and light, into oxygen and fuel. This cycle is not merely an auspicious result of billions of years of evolution. The molecular events that allow the processes of respiration and photosynthesis to happen are connected in deep ways, down to shared structures, molecules, and mechanisms. At their most basic, respiration and photosynthesis are Nature's way to capture and convert energy from one form to another. To do this, Nature has evolved complex structures, termed oxidoreductases, that bind molecules that aid in this conversion. These molecules can both absorb light, imparting plants with their colours, and take and give electrons. The oxidoreductases have evolved to take energy from external sources and convert it into forms that can be used by living organisms to grow and survive. The evident complexity of this process belies a central feature of the oxidoreductases involved: evolution has yielded structures that are built from repeats of relatively simple modules. All of respiration and photosynthesis are built on these repeating modules. But despite nearly a century of investigation, where we have outlined how respiration and photosynthesis work in fine detail, we remain unable to construct our own models of these processes. This naturally leads to a question of whether we really understand how these processes occur. Here we have assembled a team of researchers from multiple academic institutions and disciplines to address deficiencies in our knowledge, with the unified target of building completely new oxidoreductases from scratch. Through this work we will fill holes in our understanding of how Nature captures and converts energy. Our work begins by combining powerful computational techniques that allow us to design and construct oxidoreductases with tailor made functions. Within a virtual reality framework that we are developing for this project, we will work together in a shared digital space to construct molecular binding sites, alter how molecules take and give electrons or catalyse reactions, and create oxidoreductase modules that, taking inspiration from Nature, we will join to produce more complex functions. With these designs, we will use an iterative 'build-test-learn' approach to construct new oxidoreductases that match the activities and actions of those Nature uses in respiration and photosynthesis. By pulling together our expertise in computational biophysical methods, oxidoreductase engineering, modular structure creation, molecular binding site assembly and their chemistry, and the analysis of very fast oxidoreductase functions, our team stands tomake asubstantial leap in our understanding of how to construct new oxidoreductases that has, so far, remained beyond our grasp. The principles we establish through this work will help us to better understand the oxidoreductases of respiration and photosynthesis, finally clarifying architectural features that are essential for their assembly and function that have remained opaque for over a century. With our new sets of design principles, we will be able to create oxidoreductases that fulfil our needs in bioscience and biotechnology, from the creation of single structures that produce fuels from light, water and carbon dioxide akin to photosynthesis to biosensors that detect toxins in the environment or signs of disease.
Publications (none)
Final Report (none)
Added to Database 28/09/22