Projects
Projects: Projects for Investigator |
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| Reference Number | BB/G016240/1 | |
| Title | BBSRC Sustainable Bioenergy Centre: Cell wall sugars programme | |
| Status | Completed | |
| Energy Categories | Renewable Energy Sources(Bio-Energy, Other bio-energy) 60%; Renewable Energy Sources(Bio-Energy, Production of other biomass-derived fuels (incl. Production from wastes)) 20%; Renewable Energy Sources(Bio-Energy, Production of transport biofuels (incl. Production from wastes)) 20%; |
|
| Research Types | Basic and strategic applied research 100% | |
| Science and Technology Fields | BIOLOGICAL AND AGRICULTURAL SCIENCES (Biological Sciences) 50%; PHYSICAL SCIENCES AND MATHEMATICS (Chemistry) 50%; |
|
| UKERC Cross Cutting Characterisation | Not Cross-cutting 100% | |
| Principal Investigator |
Prof P (Paul ) Dupree No email address given Biochemistry University of Cambridge |
|
| Award Type | Research Grant | |
| Funding Source | BBSRC | |
| Start Date | 26 January 2009 | |
| End Date | 25 January 2014 | |
| Duration | 60 months | |
| Total Grant Value | £1,714,681 | |
| Industrial Sectors | Pharmaceuticals and Biotechnology | |
| Region | East of England | |
| Programme | Bioenergy Initiative (BEN) | |
| Investigators | Principal Investigator | Prof P (Paul ) Dupree , Biochemistry, University of Cambridge (99.997%) |
| Other Investigator | Prof J (John ) Dennis , Chemical Engineering, University of Cambridge (0.001%) Prof K (Kathryn ) Lilley , Biochemistry, University of Cambridge (0.001%) Dr J (Julian ) Griffin , Biochemistry, University of Cambridge (0.001%) |
|
| Web Site | ||
| Objectives | University of Cambridge | |
| Abstract | 1) We will develop enabling technologies for polysaccharide analysis. The field does not have the methods for high resolution screening, at the level of individual polysaccharide structures, of large populations of plants for natural or induced variation in polysaccharide quality and quantity. We also need to be able to study more precisely the specificity of hydrolytic enzyme actions. Our current techniques of PACE and LC-MS will be extended in scope, robustness and speed in this programme. 2) We will study hemicellulose degradation pathways and novel hydrolytic enzyme action. Enzymes required to remove effectively the extensive sugar decorations on branched xylan are largely unknown. We will discover and study novel debranching enzyme action, assisted by using the new profiling technologies. 3) We will use a systems approach to understanding control of polysaccharide quantity and quality. We will integrate transcriptomic and proteomic data on Golgi polysaccharide synthesis proteins to discover the enzymes, pathways and control of branched xylan and other polysaccharide synthesis. By studying polysaccharides in multiple mutants in a high throughput fashion, using our profiling technologies, we will achieve a broader understanding of polysaccharide function and the control of synthesis. 4) We will assess the consequences, for depolymerisation by the various enzymes, of altering the biosynthesis of specific branched xylan structures. 5) Using the knowledge of biosynthetic pathways, polysaccharide structure and hydrolytic enzyme action, we will propose how to select or generate crop plants with the structure of the branched xylan optimised for maximal potential sugar yield with minimal enzyme input, to ensure maximal degradation and utilisation of the biomass. | |
| Publications | (none) |
|
| Final Report | (none) |
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| Added to Database | 30/09/13 | |
