Projects
Projects: Summary of Projects by Funding SourceProjects awarded by BBSRC involving University of York : BB/D006856/1 |
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| Reference Number | BB/D006856/1 | |
| Title | Metabolomic and transcriptomic analysis of the controls on carbon partitioning into TAG reserves in oilseeds | |
| Status | Completed | |
| Energy Categories | Not Energy Related 75%; Renewable Energy Sources(Bio-Energy, Production of transport biofuels (incl. Production from wastes)) 25%; |
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| Research Types | Basic and strategic applied research 100% | |
| Science and Technology Fields | BIOLOGICAL AND AGRICULTURAL SCIENCES (Biological Sciences) 100% | |
| UKERC Cross Cutting Characterisation | Not Cross-cutting 100% | |
| Principal Investigator |
Prof IA Graham Biology University of York |
|
| Award Type | Research Grant | |
| Funding Source | BBSRC | |
| Start Date | 01 April 2006 | |
| End Date | 30 September 2009 | |
| Duration | 42 months | |
| Total Grant Value | £423,681 | |
| Industrial Sectors | Pharmaceuticals and Biotechnology | |
| Region | Yorkshire & Humberside | |
| Programme | Initiative in Plant and Microbial Metabolomics (MET) | |
| Investigators | Principal Investigator | Prof IA Graham , Biology, University of York |
| Other Investigator | Prof J Napier , Biological Chemistry and Crop Protection, Rothamsted Research Dr L Whitehead , College of Medicine, Biology and Environment, Australian National University, Australia Dr T Larson , Biology, University of York Dr O Thimm , Biology, University of York |
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| Web Site | ||
| Objectives | Objectives not supplied | |
| Abstract | This project aims to identify genes and metabolites that are key in regulating triacylglycerol (TAG) content in oilseeds. We will achieve this by analysing metabolome and transcriptome data from wild type and mutant Arabidopsis seed tissues sampled over six developmental stages that are representative of differential TAG synthesis and breakdown. Both global metabolomic analysis (NMR, GC-MS) and specialised targeted profiling methods for key lipid related metabolites such as acyl-CoAs, TAGs and sphingolipids will be performed. This will give rise to the first comprehensive metabolomic dataset for developing and germinating Arabidopsis seeds. Ten pre-selected mutants disrupted in biochemical pathways directly related to the acyl-CoA pool and/or in the regulation of TAG synthesis during seed maturation will also be profiled in the first phase of the work. Comprehensive transcriptomic datasets already exist for four of the selected seed stages and we will generate additional datsets for the two remaining stages and for two seed development stages of the abscisic acid insensitive abi3 regulatory mutant which is altered in seed maturation and storage reserve synthesis. This data will be combined with all public domain Affymetrix based transcriptomic data (currently amounting to 1,700 non-redundant datasets) in an in-house fully relational my SQL database. This database will be used to identify putative TAG related transcriptional modules and it will also be used to house the metabolomic datasets. Metabolite-specific temporal patterns across the developmental series of maturing and germinating seeds will be generated and dedicated pattern matching procedures employed to identify similarities between metabolite and transcriptomic data. Based on this approach a further set of mutants will be selected for the second phase metabolomic and targeted gene expression analysis. This research will provide a platform of knowledge that will facilitate the rational design of metabolic engineering of economically valuable oils in crop plants. | |
| Data | No related datasets |
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| Projects | No related projects |
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| Publications | No related publications |
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| Added to Database | 07/10/13 | |
