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Projects: Summary of Projects by Funding Source
Projects awarded by BBSRC involving University of York : BB/M029182/1
Reference Number BB/M029182/1
Title 14CONFAP Understanding Cell Wall Structure and Hydrolysis of Two Leading C4 Bioenergy Crops to Improve Second Generation Bioethanol Production
Status Completed
Energy Categories Not Energy Related 50%;
Renewable Energy Sources(Bio-Energy, Other bio-energy) 50%;
Research Types Basic and strategic applied research 100%
Science and Technology Fields BIOLOGICAL AND AGRICULTURAL SCIENCES (Biological Sciences) 100%
UKERC Cross Cutting Characterisation Not Cross-cutting 100%
Principal Investigator Dr LD Gomez
Biology
University of York
Award Type Research Grant
Funding Source BBSRC
Start Date 01 January 2015
End Date 31 March 2016
Duration 15 months
Total Grant Value £20,285
Industrial Sectors
Region Yorkshire & Humberside
Programme Newton Fund - Brazil (NFB)
 
Investigators Principal Investigator Dr LD Gomez , Biology, University of York
Web Site
Objectives This grant is linked to BB/M029212/1.
Abstract WP1: Tailored enzyme cocktails matching feedstock & pretreatment.
The aim will be to provide preliminary data on the potential of cocktail-feedstock-pretreatment matching. Commercial bagasse samples will undergo different pretreatments and hydrolysis with tailored enzyme cocktails and a commercial cocktail as reference. Pretreatments and tailored cocktail compositions will be selected based on expertise and scoping exercises.
WP2: Engineering the hydrolytic enzymes and feedstocks of the future.
Synthetic biology offers exciting opportunities to facilitate the release of sugars from plant biomass. The Dr Buckeridge group has fully characterized a cell wall modification system active during gas space (aerenchyma) formation in sugarcane root development. We will seek to provide proof of concept that by increasing cell wall separation and porosity with enzymes specific for grass walls, a biological pretreatment could lead to an increase in accessibility to the other polymers of the cell wall. We will also organize a workshop to effectively capture the potential of synthetic biology approaches.
WP3: Effect of environmental and genetic factors on cell wall biomass quality and conversion.
The composition and architecture of cell wall biomass can differ significantly depending on tissue, species, cultivar, and environmental conditions. We will utilize facilities at the National Plant Phenomics Centre (NPPC-Aberystwyth) to begin assessment of this variation. Selected sugarcane and Miscanthus varieties will be grown in different soil types to mimic fertile, semi-fertile and poor conditions. Plants will undergo detailed phenotyping in the NPCC during different water treatments (severe drought, moderate drought and well watered). Harvested samples will undergo cell wall phenotyping. The integration of data will provide essential information on how differences in biomass tissue, genetics, and environmental conditions impact on cell wall quality and biomass deconstruction.
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Added to Database 08/04/16